Germany Jan-Lennard Struff has also withdrawn from the US Open, the USTA said.
Kyrgios had a surgery on his left knee earlier this year and lost in his comeback match after a five-month layoff against China's Wu Yibing
Australian Open organisers said "tennis' most famous frenemies" will play each other at Rod Laver Arena on Friday.
Nine of the world's top-10 men and nine of the top-10 women have officially entered into the U.S. Open's revamped mixed doubles event, tournament organisers said on Tuesday, with former singles champions Carlos Alcaraz and Emma Raducanu teaming up.
Nick Kyrgios ended a three-year title drought with a dominant victory over Yoshito Nishioka at the Citi Open on Sunday and the Australian said he was stunned at his "incredible transformation" over the last year.
Wimbledon finalist Nick Kyrgios is one match away from a first Grand Slam title but his brother, Christos, said on Sunday the Australian is already a champion in his family's eyes after winning an off-court battle with his inner demons.
Boris Becker said Daniil Medvedev should seek professional help in the wake of his "public meltdown" during Sunday's US Open first-round defeat by Benjamin Bonzi.
Nick Kyrgios produced a serve-and-volley masterclass to topple world number one Daniil Medvedev 6-7(2), 6-4, 6-2 for his eighth straight win on a day of upsets, while American Tommy Paul rallied to beat Spanish second seed Carlos Alcaraz.
Nick Kyrgios has previously said he feels under-appreciated in his home country despite drawing crowds and media attention whenever he plays.
Kyrgios, who opted out of Australia's Davis Cup team this year, later posted a video on Instagram where he was seen out in the sunshine at a harbour with the caption 'Beautiful day'.
Nick Kyrgios will reveal in upcoming episodes of a Netflix documentary that he spent time in a psychiatric hospital.
Fiery Australian Nick Kyrgios has been fined $7,500 for "spitting and audible obscenities" during his second-round win at the US Open on Wednesday.
In a wide-ranging interview, Kyrgios discussed his image in the game and his relationship with the media, as well as revisiting the mental health issues that had him contemplating suicide in 2019.
Australian media said the charge was related to a complaint made by Kyrgios's former partner Chiara Passari.
Kyrgios was hailed for the brilliance of his tennis in the opening set but panned for his erratic behaviour
Former Mumbai Indians and Royal Challengers Bengaluru Tymal Mills has joined social media platform OnlyFans -- a subscription-based site known for hosting adult content.
Kyrgios was due to play Spain's Roberto Bautista Agut in the first round in Rome, but was replaced by lucky loser Alexandr Dolgopolov in the men's draw.
Wimbledon runner-up Nick Kyrgios does not require a coach but someone like Sigmund Freud to sort out his problems as tennis needs the Australian maverick, seven-times major winner John McEnroe said.
'I feel quite embarrassed as an Australian athlete that's seen what this guy has done for us and the sport'
'I was so close to winning a Grand Slam. I want to make sure that my body is going to have the time it needs to come back, so please bear with me.'
The 45th ranked Kyrgios came back from a set down to beat Serbia's Laslo Djere 5-7, 7-6(1) 7-6(1) on Tuesday and was scheduled to play Spaniard Roberto Bautista Agut next.
Scattered fans in the open-air stadium, at best a quarter full, wore puffer jackets and blankets, clearly backing the home favourite with occasional cheers and fist bumps.
"I'm just devastated obviously," Kyrgios told reporters
Novak Djokovic said he would make good on his deal to buy Nick Kyrgios drinks and dinner after beating the Australian to claim a fourth successive Wimbledon title on Sunday.
Novak Djokovic overcame a slow start to outclass Nick Kyrgios 4-6, 6-3, 6-4, 6-6 (7) in the final and win his seventh Wimbledon title in London on Sunday.
Images from the men's singles action at the Wimbledon Championships on Saturday.
Evert said she has been impressed by what she has seen from the 27-year-old.
Nick Kyrgios could not resist poking his nose at authority and Wimbledon's all-white clothing rules.
Nick Kyrgios's hopes of ending his country's 47-year wait for a homegrown men's champion at the Australian Open were shattered on the opening day on Monday when he pulled out of the tournament with a knee injury.
Nick Kyrgios was given a warning for taking too long to return Tsitsipas's serve
Australian government officials have said unvaccinated players will be barred from the tournament in January, casting doubt on whether men's world number one and reigning champion Novak Djokovic will defend his title.
Australian Nick Kyrgios served like a demon to bounce Serbia's world number two Novak Djokovic out of the quarter-finals of the Acapulco International with a 7-6(9), 7-5 victory on Friday.
Nick Kyrgios made it clear girlfriend Costeen Hatzi is a big reason why he feels he has come of age.
Kyrgios has been playing the best tennis of his career over the past couple of months
Kyrgios was raging after losing a challenge on his first serve and then smashed down an unstoppable, untouchable second.
It took only minutes for Kyrgios to ignite the terraces by breaking Broady in the opening game. He closed out the next game with an under-arm "tweener".
Nick Kyrgios is providing commentary by the court for ESPN and trading barbs with critics on social media. The Australian has not been shy to express an opinion.
Australian controversial tennis star Nick Kyrgios has announced that he will call curtains on his career if he wins the US Open, the fourth major of the season at Flushing Meadows.
Thiem, who also played in the charity event but did not contract the virus, told Austrian media that Kyrgios had "made a lot of mistakes" and should not criticise others.
Nick Kyrgios would rather wear all black when competing at Wimbledon instead of adhering to the all-white dress code